نتایج جستجو برای: Illumina paired-end sequencing
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The transcriptome of triangle sail mussel Hyriopsis cumingii (Lea) using Illumina paired-end sequencing technology was conducted and analyzed. Equal quantities of total RNA isolated from six tissues, including gonad, hepatopancreas, foot, mantel, gill and adductor muscle, were pooled to construct a cDNA library. A total of 58.09 million clean reads with 98.48 % Q20 bases were generated. Cluster...
SSR_pipeline is a flexible set of programs designed to efficiently identify simple sequence repeats (e.g., microsatellites) from paired-end high-throughput Illumina DNA sequencing data. The program suite contains 3 analysis modules along with a fourth control module that can automate analyses of large volumes of data. The modules are used to 1) identify the subset of paired-end sequences that p...
The transcriptome of triangle sail mussel Hyriopsis cumingii (Lea) using Illumina paired-end sequencing technology was conducted and analyzed. Equal quantities of total RNA isolated from six tissues, including gonads, hepatopancreas, foot, mantel, gills and adductor muscles, were pooled to construct a cDNA library. A total of 58.09 million clean reads with 98.48 % Q20 bases were generated. Clus...
Each artificial dataset consists of 100 000 singleor paired-end reads and was simulated using Mason v0.1.1 [1] from the Human genome (hg19), excluding haplotypes, random contigs, and ‘non-chromosomal’ sequences. For the single-end Illumina datasets, Mason was run in Illumina mode with parameters -hn2, -sq, and the read length (-n) set to 100 and 30 for long and short reads, respectively. For th...
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